Api 20 lab report

No anaerobes isolated Limitations: Transport method must be suitable for anaerobic conditions or organisms may not be viable for testing.

Api 20 lab report

Wednesday, November 16, Holly Lawson — white Title: The general principle of the experiment is to use a battery of biochemical tests for identification of enteric bacteria.

This testing system consists of a strip containing 20 chambers, each consisting of a microtube and a cupule to allow testing of 20 different tests nearly simultaneously. The method used for the API analytical profile index 20E System was the aseptic technique was used for the inoculating loop to get a unknown colony from the streak plate or pure culture slant.

API – The EPM Lab

A small amount was smeared over the filter paper and several drops of oxidase reagent were added and the color change was noted. Another loop-full of bacteria was added to the test tube containing 5 ml of sterile saline, recapped and shaken by flicking.

A Pasteur pipette was then filled with a bacterial suspension. The lid was then placed on the incubation tray and incubated at 37 degrees Celsius for hours.

An isolation streak was made on the TSA plate with a portion of the bacterial suspension to ascertain the purity of the suspension.

Api 20 lab report

After hours of incubation, all reactions not requiring addition of reagents were observed and recorded. The microtubes that required additional reagents had them added and time allotments were fulfilled to get results of remaining tests. The seven-digit profile number was then determined after all reactions were recorded on the report sheet and the profile number of the enteric unknown was calculated.

The identity of the enteric unknown was then looked up by using the API 20E software on the computer. The strip is read by noting color changes after the various indicator systems have been affected by the metabolites or added reagents.

In conclusion, the unknown enteric bacterium was identified as Serratia marcescens with a seven-digit profile number of The Nitrate reduction test was negative for gas therefore positive for Nitrate reduction. The Catalase test was also positive breaking down the H2O into O2.Laboratory Report for Groundwater & Environmental Services, Inc.

Blair Drive Suite A Laboratory Data Report: Groundwater & Environmental Services, Inc. Danielle Rice Blair Drive Suite A 4/20/ 05/04/ (EPA . These are the learning objectives, not the actual experiment objectives!LAB # Animal Behavior.

Objectives: To learn how to properly compose an AP lab report. To observe a living organism’s behavior and to design an experiment to investigate their responses to environmental variables. Introduction: Ethology is the study of animal behavior.

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AUDIT REPORT CONFIDENTIAL Company A Anytown, USA Dates of Audit: July , Date of Report: August 28, capacity to produce 20 MM lbs/yr crystalline Product B using melt crystallization technology. The The laboratory does not control the issuance or retention of laboratory .

Sample Lab Assignment. Below is a sample lab report assignment from a UW-Madison bacteriology course. We will be using a format for the lab reports which is similar (but modified) to .

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